Role of interleukin-6 in orthodontically induced inflammatory root resorption in humans
Kunii Ryuichi, Yamaguchi Masaru, Tanimoto Yasuhiro, Asano Masaki, Yamada Kunihiko, Goseki Takemi, Kasai Kazutaka,
소속 상세정보
( Kunii Ryuichi ) - Japan Nihon University School of Dentistry at Matsudo Department of Orthodontics
( Yamaguchi Masaru ) - Japan Nihon University School of Dentistry at Matsudo Department of Orthodontics
( Tanimoto Yasuhiro ) - Japan Nihon University School of Dentistry at Matsudo Department of Dental Biomaterials
( Asano Masaki ) - Japan Nihon University School of Dentistry at Matsudo Department of Orthodontics
( Yamada Kunihiko ) - Japan Nihon University School of Dentistry at Matsudo Department of Orthodontics
( Goseki Takemi ) - Japan Nihon University School of Dentistry at Matsudo Department of Orthodontics
( Kasai Kazutaka ) - Japan Nihon University School of Dentistry at Matsudo Department of Orthodontics
KMID : 0361920130430060294
Abstract
Objective: To determine the interleukin (IL)-6 levels in gingival crevicular fluid (GCF) of patients with severe root resorption after orthodontic treatment and investigate the effects of different static compressive forces (CFs) on IL-6 production by human periodontal ligament (hPDL) cells and the influence of IL-6 on osteoclastic activation from human osteoclastic precursor (hOCP) cells in vitro.
Methods: IL-6 levels in GCF samples collected from 20 patients (15 and 5 subjects without and with radiographic evidence of severe root resorption, respectively) who had undergone orthodontic treatment were measured by ELISA. The levels of IL-6 mRNA in hPDL cells and IL-6 protein in conditioned medium after the application of different uniform CFs (0, 1.0, 2.0, or 4.0 g/cm2 for up to 72 h) were measured by real-time PCR and ELISA, respectively. Finally, the influence of IL-6 on mature osteoclasts was investigated by using hOCP cells on dentin slices in a pit-formation assay.
Results: Clinically, the IL-6 levels were significantly higher in the resorption group than in the control group. In vitro, IL-6 mRNA expression significantly increased with increasing CF. IL-6 protein secretion also increased in a time- and magnitude-dependent manner. Resorbed areas on dentin slices were significantly greater in the recombinant human IL-6-treated group and group cultured in hPDL cell-conditioned medium with CF application (4.0 g/cm2) than in the group cultured in hPDL cell-conditioned medium without CF application.
Conclusions: IL-6 may play an important role in inducing or facilitating orthodontically induced inflammatory root resorption.
키워드
Interleukin-6; Osteo/odontoclastogenesis; Orthodontic force; Root resorption
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